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首页> 外文OA文献 >Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus1
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Phloem Transport of d,l-Glufosinate and Acetyl-l-Glufosinate in Glufosinate-Resistant and -Susceptible Brassica napus1

机译：耐草铵膦和感病甘蓝型油菜中d，l-草铵膦和乙酰基-l-草铵膦的韧皮部转运

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摘要

Phloem transport of d,l-[14C]glufosinate, d-[14C]glufosinate, and acetyl-l-[14C]glufosinate was examined in the susceptible Brassica napus cv Excel and a glufosinate-resistant genotype (HCN27) derived by transformation of cv Excel with the phosphinothricin-N-acetyltransferase (pat) gene. Considerably more 14C was exported from an expanded leaf in HCN27 than in cv Excel following application of d,l-[14C]glufosinate (25% versus 6.3% of applied, respectively, 72 h after treatment). The inactive isomer, d-glufosinate, was much more phloem mobile in cv Excel than racemic d,l-glufosinate. Foliar or root supplementation with 1 mm glutamine increased d,l-[14C]glufosinate translocation in cv Excel but only transiently, suggesting that glutamine depletion is not the major cause of the limited phloem transport. Acetyl-l-[14C]glufosinate (applied as such or derived from l-glufosinate in pat transformants) was translocated extensively in the phloem of both genotypes. Acetyl-l-[14C]glufosinate was readily transported into the floral buds and flowers, and accumulated in the anthers in both genotypes. These results suggest that phloem transport of d,l-glufosinate is limited by rapid physiological effects of the l-isomer in source leaf tissue. The accumulation of acetyl-l-glufosinate in the anthers indicates that it is sufficiently phloem mobile to act as a foliar-applied chemical inducer of male sterility in plants expressing a deacetylase gene in the tapetum, generating toxic concentrations of l-glufosinate in pollen-producing tissues.
机译：在易感的甘蓝型油菜cv Excel中检测了d，1- [14C]草铵膦，d- [14C]草铵膦和乙酰基-1- [14C]草铵膦的韧皮运输和通过转化草甘膦而产生的抗草铵膦基因型（HCN27）具有草丁膦-N-乙酰基转移酶（pat）基因的cv Excel。在施用d，1- [14C]草铵膦之后，从HCN27中的膨胀叶片中输出的14C比cv Excel中输出的要多得多（处理后72小时分别施用25％对6.3％）。非活性异构体d-草铵膦比消旋的d，l-草铵膦在cv Excel中的韧皮部流动性高得多。叶面或根部补充1 mm谷氨酰胺会增加cv Excel中d，1- [14C]草铵膦的移位，但只是暂时的，这表明谷氨酰胺的消耗不是韧皮部转运受限的主要原因。乙酰基-1- [14C]草铵膦（原样应用或衍生自pat-转化体中的1-草铵膦）在两种基因型的韧皮部中广泛移位。乙酰基-1- [14C]草铵膦易于转运到花蕾和花中，并在两种基因型的花药中积累。这些结果表明，d，l-草铵膦的韧皮部转运受到源叶组织中l-异构体的快速生理作用的限制。乙酰基-1-草铵膦在花药中的积累表明它具有足够的韧皮部流动性，可在绒毡层中表达脱乙酰酶基因的植物中用作叶不育的雄性不育化学诱导剂，从而在花粉中产生有毒浓度的1-草铵膦生产组织。

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